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Alberts B, Johnson A, Lewis J, et al. Molecular Biology of the Cell. 4th edition. New York: Garland Science; 2002.

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Cover of Molecular Biology of the Cell

Molecular Biology of the Cell. 4th edition.

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Figure 9-12. The optical system of a fluorescence microscope.

Figure 9-12The optical system of a fluorescence microscope

A filter set consists of two barrier filters (1 and 3) and a dichroic (beam-splitting) mirror (2). In this example, the filter set for detection of the fluorescent molecule fluorescein is shown. High-numerical-aperture objective lenses are especially important in this type of microscopy because, for a given magnification, the brightness of the fluorescent image is proportional to the fourth power of the numerical aperture (see also Figure 9-6).

From: Looking at the Structure of Cells in the Microscope

Copyright © 2002, Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts, and Peter Walter; Copyright © 1983, 1989, 1994, Bruce Alberts, Dennis Bray, Julian Lewis, Martin Raff, Keith Roberts, and James D. Watson .

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