LOCUS       G10613                   661 bp    DNA     linear   STS 15-AUG-1995 
DEFINITION  human STS CHLC.UTR_01572_M14745.P56090 clone UTR_01572_M14745, 
            sequence tagged site. 
ACCESSION   G10613 
VERSION     G10613.1  GI:942462 
KEYWORDS    STS; STS sequence; primer; sequence tagged site. 
SOURCE      Homo sapiens (human) 
  ORGANISM  Homo sapiens 
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; 
            Mammalia; Eutheria; Primates; Catarrhini; Hominidae; Homo. 
REFERENCE   1  (bases 1 to 661) 
  AUTHORS   Murray,J., Sheffield,V, Weber,J.L., Duyk,G. and Buetow,K.H. 
  TITLE     Cooperative Human Linkage Center 
  JOURNAL   Unpublished (1995) 
COMMENT     Synonyms: UTR_01572_M14745, CHLC.UTR_01572_M14745.T36135 
            Contact: Dr. Jeffrey C. Murray 
            UofI 
            The University of Iowa 
            Department of Pediatrics, Iowa City, IA 52242, USA 
            Tel: (319) 356-3508 
            Fax: (319) 356-3347 
            Email: jeff-murray@uiowa.edu 
             
            Primer A: TGCATTGTCAGTGATGTTCC 
            Primer B: CGTTTTGCCTGAAGACTGTT 
            STS size: 127 
            PCR Profile: 
                          denature:    30 seconds at 94 degrees C 
                          annealing:   75 seconds at 55 degrees C 
                          extension:   15 seconds at 72 degrees C 
                          PCR cycles:  27 
                          extension:    6 minutes at 72 degress C 
            Protocol: 
                          Template:        30ng genomic DNA 
                          Primer:          each 1.5 pmole 
                          dNTPs:           each 200 uM 
                          Taq Polymerase:  0.3 units 
                          Total Vol:       10 ul 
            
            Buffer: 
                          MgCl2:  1.5mM 
                          KCl:     50mM 
                          Tris:    10mM 
                          pH:     8.3 
            
            Prepared Prepared with primer pairs derived from M14745. 
FEATURES             Location/Qualifiers 
     source          1..661 
                     /organism="Homo sapiens" 
                     /db_xref="taxon:9606" 
     STS             61..187 
     primer_bind     61..80 
     primer_bind     complement(168..187) 
BASE COUNT      205 a    141 c    148 g    167 t 
ORIGIN      
        1 agtcaacatg cctgccccaa acaaatatgc aaaaggttca ctaaagcagt agaaataata 
       61 tgcattgtca gtgatgttcc atgaaacaaa gctgcaggct gtttaagaaa aaataacaca 
      121 catataaaca tcacacacac agacagacac acacacacac aacaattaac agtcttcagg 
      181 caaaacgtcg aatcagctat ttactgccaa agggaaatat catttatttt ttacattatt 
      241 aagaaaaaaa gatttattta tttaagacag tcccatcaaa actcctgtct ttggaaatcc 
      301 gaccactaat tgccaagcac cgcttcgtgt ggctccacct ggatgttctg tgcctgtaaa 
      361 catagattcg ctttccatgt tgttggccgg atcaccatct gaagagcaga cggatggaaa 
      421 aaggacctga tcattgggga agctggcttt ctggctgctg gaggctgggg agaaggtgtt 
      481 cattcacttg catttctttg ccctgggggc tgtgatatta acagagggag ggttcctgtg 
      541 gggggaagtc catgcctccc tggcctgaag aagagactct ttgcatatga ctcacatgat 
      601 gcatacctgg tgggaggaaa agagttggga acttcagatg gacctagtac ccactgagat 
      661 t 
//